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About SpringerProtocols
SpringerProtocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences. Compiling protocols from Humana’s successful book series Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, and Neuromethods, as well as from a vast number of Laboratory Handbooks, such as The Biomethods Handbook, The Proteomics Handbook, and the Springer Laboratory Manuals, SpringerProtocols offers researchers access to nearly thirty years worth of time tested, easily reproducible, step-by-step protocols for immediate use in their lab. Written by renowned experts in the field with an emphasis on both comprehensiveness and ease of use, each protocol is organized in an easily reproducible recipe style, and offering helpful tricks of the trade in an invaluable “notes” section, SpringerProtocols is an invaluable resource for the modern research laboratory. All SpringerProtocols are written by experts in the field identified and reviewed by distinguished editors in their specific subject areas. Prior to publication each protocol is reviewed for clarity, accuracy, and consistency by the subject editor, a member of our prestigious editorial board, and Springer's in-house editorial staff. Video Presentation: What Is SpringerProtocols? Contact Us For technical support or customer service: In the Americas Springer Science & Business Media SpringerLink Helpdesk P.O Box 2485 Secaucus, N.J.07094-2485 U.S.A Tel: 1–888–777–4643 (Weekdays 8:30am–5:30pm ET) Fax: +1 201–255–7017 E-mail: springerlink-ny@springer.com All Other Countries Springer Distribution Center (SDC) Haberstr. 7 D-69126 Heidelberg Germany Tel: ++49 6221 345–4303 (Weekdays 8:00am–8pm) Fax: ++49 6221 345–4229 E-mail: springerlink@springer.com For sales inquiries: Academic Libraries The Americas Europe & Africa Asia & Australia Corporate & Govt. Libraries The Americas Europe & The Rest of the World

About Humana Press

Humana Press is a major, worldwide publisher of books and journals in molecular biology, neuroscience, cancer research, and medicine.  Humana publishes approximately 150 book titles per year, roughly half in Clinical Medicine and half in Biomedicine / Life Sciences. Humana also has a distinguished backlist of approximately 1,700 titles and a portfolio of 25 journals. Humana is perhaps best known for its Methods series, including Methods in Molecular Biology, Neuromethods, Methods in Pharmacology and Toxicology, and Methods in Biotechnology series. The Methods series are recognized and renowned for the step-wise “recipe” format that makes all the protocols readily reproducible and a troubleshooting section that includes hints and tips on common mistakes. Humana’s Cancer Drug Discovery and Development series is also a mainstay of its Biomedicine/Life Sciences publishing program.  One of Humana’s most successful products is the industry-leading online protocol database, SpringerProtocols.com. Humana’s clinical series include Contemporary Cardiology and Current Clinical Practice, Contemporary Endocrinology, Contemporary Diabetes, Current Clinical Urology, Current Clinical Oncology, Forensic Science and Medicine, and Nutrition and Health. Humana also publishes a number of successful non-series titles each year, including the major reference work, Principles of Molecular Medicine.

SpringerProtocols Executive Editorial Board

Editor in Chief John M. Walker, University of Hertfordshire

Plant Science Kan Wang, Iowa State University

Genetics/Genomics Mike Starkey, Animal Health Trust

Cancer Research Paul Fisher, Virginia Commonwealth University

Cell Biology Kursad Turksen, Ottawa Health Research Institute

Pharmacology and Toxicology Y. James Kang, University of Louisville

Neuroscience Wolfgang Walz, University of Saskatchewan, College of Medicine