SpringerProtocols: Abstract: A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors

A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors

By: Michael Rau², Theophile Ohlmann², Virginia M. Pain², Simon J. Morley²

Abstract

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The supplemented rabbit reticulocyte lysate is one of the most active cell-free translation systems known and has been extensively used over the last 20 yr or so for in vitro translation of endogenous (globin), as well as of heterologous mRNAs. In addition, it has regularly served as the starting material for the purification, identification, and indeed the characterization of some of the many protein factors required for eukaryotic protein synthesis.

Affiliation(s): (2) Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Brighton, UK

Book Title: Protein Synthesis: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 77 | Pub. Date: Aug-04-1998 | Page Range: 211-226 | DOI: 10.1385/0-89603-397-X:211

Subject: Protein Science

References

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