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| Abstract |
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Human skin is a rich source of human antimicrobial peptides. Its cellular source is the keratinocyte, which terminally differentiates
in the uppermost parts of the skin, eventually forming the stratum corneum, the horny layer. The easy availability of human
stratum corneum makes it possible to identify and characterize human antimicrobial peptides with a biochemical approach. Moreover,
the availability of lesional scales of patients with psoriasis, an inflammatory skin disease, allows the identification of
human-inducible peptide antibiotics, which are absent in healthy skin. With this strategy, the beta-defensins hBD-2 and hBD-3,
RNase-7 as well as psoriasin/S100A7 have been discovered as human antimicrobial peptides and proteins.
A detailed description of the strategies and methods is presented, which allowed a successful identification and characterization
of human antimicrobial peptides. We used various HPLC techniques, combined with antimicrobial testing as read-out system.
In parallel, SDS-PAGE analyses as well as electrospray ionization mass spectrometry were used for further biochemical characterization
as well as purity assessment.
Affiliation(s): (1) Clinical Research Unit “Cutaneous Inflammation”, Department of Dermatology, University Hospital Schleswig-Holstein, Kiel, Germany
Book Title: Antimicrobial Peptides: Methods and Protocols
Series: Methods in Molecular Biology | Volume: 618 | Pub. Date: Jan-22-2010 | Page Range: 15-30 | DOI: 10.1007/978-1-60761-594-1_2
Subject: Immunology
Key Words: Antimicrobial peptides - defensin - RNase-7 - S100A7 - skin - stratum corneum - purification - electrospray ionization mass spectrometry - high-performance liquid chromatography
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