Springer Protocols: Abstract: 38. Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses

38. Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses

By: Zarir E. Vaghchhipawala⁶, Kirankumar S. Mysore⁶

Abstract

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Plant-virus interaction studies, for long, plagued by asynchronous/failed infections, have improved since the usage of Agrobacterium as a delivery agent for viral genomes. Popularly known as “agroinoculation,” this method has revolutionized plant virology studies, leading to identification of viruses as casual agents of disease, viral genome mutagenesis and recombination analyses, and virus-induced gene silencing (VIGS) applications. We present here a brief overview of the recent applications of this method and a detailed protocol for agroinoculation and VIGS used in our laboratory.

Affiliation(s): (6) Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pky., Ardmore, OK 73401, USA

Book Title: Plant Virology Protocols: From Viral Sequence to Protein Function

Series: Methods in Molecular Biology | Volume: 451 | Pub. Date: Apr-01-2008 | Page Range: 555-562 | DOI: 10.1007/978-1-59745-102-4_38

Subject: Plant Sciences

Key Words: Agrobacterium - Agroinoculation - Agroinfiltration - Agrodrench - VIGS - Tobacco rattle virus (TRV) vector

References

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