Springer Protocols: Abstract: Biosensors for RNA Aptamers

Biosensors for RNA Aptamers—Protein Interaction

By: Sara Tombelli³, Maria Minunni³, Marco Mascini³

Abstract

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Aptamers are an alternative to antibodies in their role as biorecognition elements in analytical devices. RNA aptamers, specific for different proteins, have been exploited as biorecognition elements to develop specific biosensors (aptasensors). These recognition elements have been coupled to piezoelectric quartz crystals and surface plasmon resonance (SPR) devices as transducers. The procedure for fixing the aptamer onto these transducers and for monitoring the interaction with the target protein is shown here.

Affiliation(s): (3) Dipartimento di Chimica, Università degli Studi di Firenze, Sesto Fiorentino, Italy

Book Title: Post-Transcriptional Gene Regulation

Series: Methods in Molecular Biology | Volume: 419 | Pub. Date: Dec-21-2007 | Page Range: 109-119 | DOI: 10.1007/978-1-59745-033-1_7

Subject: Genetics/Genomics

Key Words: RNA - aptamers - Tat protein - biosensor - quartz crystal microbalance - surface plasmon resonance

References

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